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Authors
Affiliations
1 Research Department of Plant Biology and Biotechnology, Loyola College, Chennai - 600 034, IN
Source
South Indian Journal of Biological Sciences, Vol 1, No 1 (2015), Pagination: 52-59
Abstract
This study was conducted to develop an efficient protocol for in vitro direct and indirect regeneration of Epaltes divaricata L. MS (Murashige and Skoog) medium was supplemented with different growth hormones both individually and in combinations. MS medium containing BAP (1.0 mg/L) and TDZ (0.1 mg/L) in combination proved to be most effective in inducing maximum callus with multiple shoot formation using shoot tip from the explants producing maximum of 10.77±0.4 shootlets / explants with mean length of 4.30±0.3 cm from leaf explants. Regenerated multiple shoot induction using nodal explants supplemented BAP (1.0 mg/l) with NAA (0.1 mg/L) produced 1.86±1.05 average number of shoots / explants, with mean length of shoots 3.00±0.64 cm whereas BAP (1.0 mg/L) with KN (0.1 mg/L) produced 4.16±0.36 average number of shoots with mean length of shoot 3.08±0.33 cm. The leaf explants produced good callus in the combination of growth regulator BAP (0.5 mg/L) and NAA (0.1 mg/L) 80% response and number of shoot per callus is 3.38 ± 0.21. In vitro ischolar_maining of micro propagated shoot response in MS media with IBA (0.1 mg/L) producing more ischolar_mains (13.31±0.24 numbers) with ischolar_main length 7.16±0.51 cm followed by NAA (0.1 mg/L) producing 8.21±0.26 numbers with ischolar_main length 5.22±0.41 cm. Regenerated of Epaltes divaricata L. plantlets were hardened in organic manure with sand showed acclimatization percentage is 73.33 % after 2 months in the field.
Keywords
MS Medium, Multiple Shoots, Regeneration, Hardening, Epaltes divaricata L.
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